Product Name 

BrdU antibody [BU1/75 (ICR1)]

Product type 

Primary antibodies

Description 

Rat monoclonal [BU1/75 (ICR1)] to BrdU

Immunogen 

The details of the immunogen for this antibody are not available.

Species Reactivity 

Not applicable.
 

Specificity 

This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine.
The antibody does not cross react with IdU.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-FoFr, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/40.
ICC/IF: 1/250
IHC-P: 1/40. It is strongly recommended to use an amplification method such as ABC or PAP/APAAP to visualize staining.
IHC-Fr: 1/40 - 1/200. In addition, found to work at 1/400 (see PMID: 16670699).
Immunohistochemistry (PFA fixed): 1/40 (from PubMed:16373695).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody is also available in FITC (ab74545), HRP (ab74546) and Biotin (ab74547) conjugated formats.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers

Relevance 

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.

BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

BU1/75 (ICR1)

Isotype 

IgG2a

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
 

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results.

See below for BrdU antibody [BU1/75 (ICR1)] images, references, products related to ab6326 and other tools.