产品编号:P119-50
产品描述:Recombinant Human Pleiotrophin(PTN)
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4275.00
订购:
说明书:
Recombinant Human Pleiotrophin (PTN)
Purified No Carrier Protein
(PTN, HARP, Heparin Binding Growth Factor 8 (HBGF8), HBNF, Neurite Growth-Promoting Factor 1 (NEGF1))
Prod. No.: P119
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 50 µg, 250 µg
Storage: -20°C to -70°C
Description
Background:
Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), also known as PTN is a growth, differentiation inducing cytokine,1 and neurite extension promoting polypeptide which is highly expressed in brain and in tissues derived from mesenchyme.2 PTN is produced by many different human cancers and stimulates tumor blood vessel formation when it is expressed in malignant cancer cells.3 It is expressed in many types of cancer, such as gliomas, melanomas, meningiomas, neuroblastomas, choriocarcinomas, leukemias and cancers of pancreas, prostate, stomach, colon, breast, ovaries and lungs.4
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human PTN is Mr 15.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 18 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human PTN was determined by its ability to enhance neurite outgrowth of cerebral cortical neurons of E10 chick embryos (Hampton, B.S. et al., 1992, Mol. Biol. Cell 3:85 - 93). Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 μL/well of a solution of 3 - 8 μg/ml rhPTN.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
References
1. Lee RJ et al. (2005) Biochem Biophys Res Commun. 332: 1146
2. Deuel TF et al. (1992) Biochem Biophys Res Commun. 184: 427
3. Berenson JR et al. (2009) Blood 113: 1992
4. Papadimitriou E. et al. (2007) Recent Patents Anticancer Drug Discov. 2: 175
Related Products
Anti-Human Pleiotrophin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: P289)
