产品编号:M130-1
产品描述:Recombinant Human MIP-4
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Macrophage Inflammatory Protein-4 (MIP-4)
Purified No Carrier Protein
(Pulmonary and Activation-Regulated Chemokine (PARC), SCYA18, DC-CK1, AMAC-1, DCCK1, CKb7, CCL18)
Prod. No.: M130
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage Inflammatory Protein-4 (MIP-4) produced in E.Coli is a single, non-glycosylated polypeptide that attracts lymphocytes but not monocytes or granulocytes and is involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. MIP-4 is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MIP-4 is Mr 7.8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MIP-4 was determined by by its ability to induce chemotaxis of resting T-lymphocytes and its ability to inhibit rhEotaxin induced chemotaxis on rat Y3 cells transfected with human CCR3 (Nibbs, RJB. et al., 2000, J. Immunol. 164:1488). The expected ED50 for these effects is typically 0.5 - 2 μg/ml and 1 - 5 μg/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
aqvgtnkelc clvytswqip qkfivdyset spqcpkpgvi lltkrgrqic adpnkkwvqk yisdlklna
References
1. Nomiyama H et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik T. et al. (2005) J. Pathol. 166: 1647
Related Products
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