产品编号:M168-1
产品描述:Recombinant Human MIP-3β
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Macrophage Inflammatory Protein-3 Beta (MIP-3β)
Purified No Carrier Protein
(CCL19, AMAC-1, Ckb7, Ck-Beta 7, DC-CK1, MIP4, PARC, SCYA18, MIP-3B, Exodus-3, EBI1-Ligand Chemokine (ELC))
Prod. No.: M168
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage inflammatory protein-3beta (MIP-3beta) is a non-glycosylated polypeptide and member of the B-chemokine (C-C) family of cytokines. It plays an important role in the inflammatory response of human T cells and tissue macrophages. MIP-3beta is a potential chemoattractant for endometrial natural killer cells.1 It may be important in trafficking of T cells in thymus, and T cell and B cell migration to secondary lymphoid organs.2
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MIP-3β is Mr 8.8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MIP-3β was determined by its ability to chemoattract cultured (5 - 10 days) human lymphocytes or human T-lymphoblastoid CEM NKr cells (Howell, D.N. et al.,1985, J. Immunol. 134:971-976). The expected ED50 is typically 0.1 - 0.3 μg/ml and 0.2 - 0.6 μg/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
gtndaedcc lsvtqkpipg yivrnfhyll ikdgcrvpav vfttlrgrql cappdqpwve riiqrlqrts akmkrrss
References
1. Honjo H. et al. (2004) Fertil Steril. 1: 876
3. Broxmeyer HE. et al. (1998) J Immunol. 160: 2418
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