产品编号:M201-1
产品描述:Recombinant Mouse MIP-3α
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Macrophage Inflammatory Protein-3 Alpha (MIP-3α)
Purified No Carrier Protein
(CCL20, CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1)
Prod. No.: M201
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyers patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse MIP-3α is Mr 7.9 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse MIP-3α was determined by its ability to chemoattract mouse BaF/3 cells transfected with hCCR6. The expected ED50
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
asny dcclsyiqtp lpsraivgft rqmadeacdi naiifhtkkr ksvcadpkqn wvkravnlls lrvkkm
References
1. Nomiyama H et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik T. et al. (2005) J. Pathol. 166: 1647
3. Cook DN. et al. (2000) Immunity 12: 495
4. Farber JM. et al. (2002) J. Immunol. 168: 4871
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