产品编号:M159-25
产品描述:Recombinant Human MCP-4
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4275.00
订购:
说明书:
Recombinant Human Monocyte Chemotactic Protein-4 (MCP-4)
Purified No Carrier Protein
(CCL13, SCYA13, NCC-1, SCYL1, CKb10)
Prod. No.: M159
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Monocyte chemoattractant protein-4 (MCP-4), also know as CCL13, is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. MCP-4 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell1,2 MCP-4 can cause joint destruction in rheumatoid arthritis.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human MCP-4 is Mr 8.6 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MCP-4 was determined by by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485) and by its ability to chemoattract hCCR2A transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 0.2 - 0.6 μg/ml and 0.5 - 2 μg/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
qpdalnv pstccftfss kkislqrlks yvittsrcpq kavifrtklg keicadpkek wvqnymkhlg rkahtlkt
References
1. Luster AD et al. (1996) J Immunol. 157: 5613
2. Schleimer RP et al. (1997) J Clin Invest. 99: 926
3. S. Momohara et al. (2005) Rheumatology 10: 1093
Related Products
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Anti-Human MCP-4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M127)
