产品编号:H1477-1
产品描述:Recombinant Mouse HGF R
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse HGF R
Purified No Carrier Protein
(C-MET, HGFR, RCCP2)
Prod. No.: H1477
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
Hepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.1 C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.2,3
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse HGF R is Mr 128 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS Page and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to bind rmHGF in a functional ELISA type binding assay with an estimated KD < 0.2 nM.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
Mouse HGF R (Met 1 - Asn 929) - Linker peptide (DIEGRMD) - Human IgG1 (Pro 100 - Lys 330) - 6x His Tag
References
1. McDonald DM. et al. (2008) BMB Rep. 41: 833
2. Park M. et al. (1998) Oncogene 16: 833
3. Comoglio PM et al. (1998) J Cell Sci. 111: 237
