产品编号:C1363-1

产品描述:Recombinant Human Cathepsin L

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:

说明书:

Recombinant Human Cathepsin L
Purified No Carrier Protein
（Ctsl1）
Prod. No.: C1363
Source NSO Cells
Pkg. Size: 1 mg, 10 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
A cathepsin is a cysteine or aspartic protease, a type of protein that breaks apart other proteins, found in many types of cells including those in all animals. There are approximately a dozen members of this family, which are distinguished by their structure ａnd which proteins they cleave. Most of the members become activated at the low pH found in lysosomes. Thus, the activity of this family lies almost entirely within those organelles. Cathepsins have a vital role in mammalian cellular turnover, e.g. bone resorption. They degrade polypeptides ａnd are distinguished by their substrate specificites.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human Cathepsin L is Mr 36 kDa.
State of Matter
Frozen
Formulation
The cytokine is supplied frozen in 50 mM sodium acetate, 0.4 M NaCl, pH 5.0.
Purity
>95% by SDS Page ａnd analyzed by silver stain.
Storage ａnd Stability
The frozen protein is stable for 6 months when kept a -20°C to -70°C in a manual defrost freezer. Avoid repeat freeze thaw cycles..
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Cathepsin L was determined by its ability to cleave a fluorescent peptide substrate, N-carbobenzyloxy-Leu-Arg-7-amido-4-methylcoumarin （Z-L-R-AMC）. The specific activity, measured with 10 μM Z-L-R-AMC ａnd 1 ng enzyme in 100 μL of 50 mM MES, 5 mM dithiothreitol, pH 6.0, at room temperature, is > 15,000 pmoles/min/μg.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
eaprsvdwre kgyvtpvknq gqcgscwafs atgalegqmf rktgrlisls eqnlvdcsgp qgnegcnggl mdyafqyvqd nggldseesy pyeateesck ynpkysvand tgfvdipkqe kalmkavatv gpisvaidag hesflfykeg iyfepdcsse dmdhgvlvvg ygfestesdn nkywlvknsw geewgmggyv kmakdrrnhc giasaasypt vhhhhhh

Country of Origin
USA
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