产品编号:C1340-200
产品描述:Recombinant Mouse CTLA-4
反应种属:
实验方法:
标记:
规格:200µg
供应商:Leinco
价格(RMB):5400.00
订购:
说明书:
Recombinant Mouse Cytotoxic T-Lymphocyte-Associated Molecule-4 （CTLA-4）
Purified No Carrier Protein
（CD152, CELIAC3, GSE, IDDM12, Ly-56）
Prod. No.: C1340
Source NSO Cells
Pkg. Size: 1 mg, 200 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
CTLA4 （Cytotoxic T-Lymphocyte Antigen 4） also known as CD152, is a protein which is expressed on the surface of Helper T cells ａnd plays an important regulatory role in the immune system.1 CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells.2,3 CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis ａnd may also be useful during organ transplantation ａnd cancer treatment.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse CTLA-4 is Mr 41 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 55 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to inhibit IL-2 production in Jurkat cells stimulated with 1 μg/ml rhB7-1/Fc in the presence of PHA （Linsley, P.S. et al., 1991, J. Exp. Med. 174:561）. The expected ED50 for this effect is 0.1 - 0.4 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
aiqv tqpsvvlass hgvasfpcey spshntdevr vtvlrqtndq mtevcattft ekntvgfldy pfcsgtfnes rvnltiqglr avdtglylck velmypppyf vgmgngtqiy vidpepcpds dfiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh
Country of Origin
USA
References
1. Layrisse Z et al. （2009） Hum Immunol.
2. Mak TW et al. （1995） Science 270: 985
3. Delneste Y et al. （1999） Eur. J. Immunol 29: 3596
4. Vijayakrishnan L et al. （2004） Immunity 20: 563
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