产品编号:E128
产品描述:Anti-Mouse CCL21-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5320.00
订购:
说明书:
Goat Anti-Mouse Chemokine (C-C Motif) Ligand 21 (CCL21)
Biotin
(6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Exodus-2)
Polyclonal Antibody
Prod. No.: E128
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Exodus-2 (CCL21) recognizes Mouse CCL21. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.
In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect.
Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse CCL21 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Related Products
Recombinant Mouse CCL21 (Leinco Prod. No.: E114)
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Anti-Human CCL21 (Antigen Aff. Pur.) (Leinco Prod. No.: E116)
Recombinant Human CCL21 (Mucin) (Leinco Prod. No.: C1365)
Anti-Human CCL21 (Leinco Prod. No.: C1407)
Anti-Mouse CCL21 (Leinco Prod. No.: C1421)
Anti-Human Exodus-2/CCL21-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E127)
Anti-Mouse CCL21-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1500)
Recombinant Human Exodus-2 (CCL21) (Leinco Prod. No.: E113)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
