产品编号:E135
产品描述:Anti-Mouse EGF-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Epidermal Growth Factor (EGF)
Biotin
(Urogastrone, URG, C-erbB)
Polyclonal Antibody
Prod. No.: E135
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Epidermal Growth Factor (EGF) recognizes Mouse EGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR.1 EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. This causes variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.2 EGF has been shown to regulate tumor cell invasion through activation of matrix metalloproteinase-2 (MMP-2) in various tumor cell types.3
Known Species Cross Reactivity: Ms+
Immunogen
E. coli-Derived Recombinant Mouse EGF
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Mouse EGF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse EGF is 5 and 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse EGF (Leinco Prod. No.: E120).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with Anti-Mouse EGF (Leinco Prod. No.: E321) as the capture antibody at 2 - 8 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Mouse EGF.
References
1. Cohen S et al. (1995) J. Biol. Chem 265: 7709
2. Seroogy KB et al. (1995) Science 224: 1107
3. Cosen-Binker LI. et al. (2009) Biochem Biophys Res Commun. 379: 445
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse EGF (Leinco Prod. No.: E120)
Anti-Mouse EGF (Leinco Prod. No.: E321)
Anti-Mouse EGF (Antigen Aff. Pur.) (Leinco Prod. No.: E134)
Recombinant Rat EGF (Leinco Prod. No.: E188)
Anti-Human EGF (Leinco Prod. No.: E239)
Anti-Human EGF (Leinco Prod. No.: E256)
Anti-Human EGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E276)
Anti-Human EGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E121)
Human EGF ELISA Development Kit (Leinco Prod. No.: E141)
Mouse EGF ELISA Development Kit (Leinco Prod. No.: E305)
Anti-Human EGF (Antigen Aff. Pur.) (Leinco Prod. No.: E104)
PN:
Recombinant Human EGF (Leinco Prod. No.: E108)
Anti-Rat EGF ( Antigen Aff. Pur. ) (Leinco Prod. No.: E315)
Anti-Rat EGF- Biotin ( Antigen Aff. Pur. ) (Leinco Prod. No.: B636)
Anti-Rat EGF (Leinco Prod. No.: E323)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
