产品编号:C452
产品描述:Anti-Mouse CXCL16-Biotin
反应种属:mouse
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 16 (CXCL16)
Biotin
(SCYB16, SR-PSOX, CXCLG16, Zmynd15, AV290116, BB024863, 0910001K24Rik)
Polyclonal Antibody
Prod. No.: C452
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse CXCL16 recognizes Mouse CXCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a transmembrane protein that facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation.1 CXCL16 is found in liver tissue2 and influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides.3 CXCL16 may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease.4
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse CXCL16 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Mouse CXCL16 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CXCL16 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Mummidi S et al. (2004) J Biol Chem. 279: 3188
2. Adams DH et al. (2005) J Immunol. 174: 1055
3. Clinman DM et al. (2006) J Immunol. 177: 1575
4. Broedl UC et al. (2008) Scand J Gastroenterol. 43: 283
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL16 (Antigen Aff. Pur.) (Leinco Prod. No.: C848)
Recombinant Mouse CXCL16 (Leinco Prod. No.: C847)
Recombinant Human CXCL16 (Leinco Prod. No.: C843)
Anti-Mouse CXCL16 (Antigen Aff. Pur.) (Leinco Prod. No.: C451)
Recombinant Human CXCL16 (Extracellular Domain) (Leinco Prod. No.: C1335)
Anti-Mouse CXCL16 (Leinco Prod. No.: C1430)
Anti-Human CXCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C849)
Anti-Mouse CXCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1505)
Human CXCL16 ELISA Development Kit (Leinco Prod. No.: C337)
Recombinant Mouse CXCL16 (Extracellular Domain) (Leinco Prod. No.: C1590)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
