产品编号:T192
产品描述:Anti-Human TNF-β-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Tumor Necrosis Factor-Beta (TNF-β)
Biotin
(Lymphotoxin-Alpha (LT Alpha), Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha 3)
Polyclonal Antibody
Prod. No.: T192
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Tumor Necrosis Factor-β (TNF-β) recognizes Human TNF-β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Tumor necrosis factor-beta (TNF beta) also known as lymphotoxin alpha (LT alpha), is a multifaceted pro-inflammatory cytokine which may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.1 TNF beta has been implicated in the pathogenesis of several autoimmune diseases and it plays a significant role in the etiopathogenesis of multiple sclerosis (MS).2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human TNF-β (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human TNF-β this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TNF-β is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human TNF-β (Leinco Prod. No.: T154).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml.
References
1. Adolf GR et al. (1990) Infec Immun. 58: 3996
2. Pandey JP et al. (2002) Autoimmunity. 35: 377
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human TNF-β (Leinco Prod. No.: T154)
Recombinant Human TNF-β (Insect Cell Derived) (Leinco Prod. No.: T238)
Anti-Human TNF-β (Antigen Aff. Pur.) (Leinco Prod. No.: T147)
Anti-Human TNF-β (Leinco Prod. No.: T546)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
