产品编号:T187
产品描述:Anti-Human TNF-α-Biotin
反应种属:human
实验方法:WB, ELISA., ICC
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Tumor Necrosis Factor-α (TNF-α)
Biotin
(Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin)
Polyclonal Antibody
Prod. No.: T187
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Tumor Necrosis Factor-α (TNF-α) recognizes Human TNF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis.
Known Species Cross Reactivity: Ms-, Hu+, Rt-
Immunogen
Purified Recombinant Human TNF-α (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB,
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human TNF-α this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TNF-α is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human TNF-α (Leinco Prod. No.: T153).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml.
References
1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2. Adolf GR et al. (1990) Infec Immun. 58: 3996
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human TNF-α (Leinco Prod. No.: T153)
Anti-Mouse TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T149)
Recombinant Mouse TNF-α (Leinco Prod. No.: T156)
Anti-Mouse TNF-α - Low Endotoxin Functional Formulation (Leinco Prod. No.: T258)
Anti-Mouse TNFα-Purified (Leinco Prod. No.: T259)
Anti-Mouse TNFα-R-phycoerythrin (Leinco Prod. No.: T262)
Human TNF-α ELISA Development Kit (Leinco Prod. No.: T200)
Recombinant Mouse TNF-α (Leinco Prod. No.: T376)
Anti-Human TNF-α (Leinco Prod. No.: T427)
Anti-Rat TNF-α (Leinco Prod. No.: T442)
Anti-Porcine TNF-α (Leinco Prod. No.: T455)
Anti-Porcine TNF-α (Leinco Prod. No.: T456)
Anti-Human TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T146)
Anti-Human TNF-α (Monoclonal Antibody) (Leinco Prod. No.: T190)
Anti-Mouse TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T188)
Anti-Rat TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T150)
Anti-Rat TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T189)
Recombinant Rat TNF-α (Leinco Prod. No.: T157)
Anti-Human Extracellular TNF-α-Fluorescein (FITC) (Leinco Prod. No.: T505)
Rat TNF-α ELISA Development Kit (Leinco Prod. No.: T518)
Mouse TNF-α ELISA Development Kit (Leinco Prod. No.: T519)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
