产品编号:T195
产品描述:Anti-Human TPO-Biotin
反应种属:human
实验方法:WB, ELISA
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Thrombopoietin (TPO)
Biotin
(THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand, Leukemia Virus Oncogene Ligand)
Polyclonal Antibody
Prod. No.: T195
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Thrombopoietin (TPO) recognizes Human TPO. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1
Known Species Cross Reactivity: Hu+
Immunogen
Sf 21-Derived Recombinant Human Tpo
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human TPO this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TPO is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Vainchenker W et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda RC et al. (1998) Blood 92: 4023
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human TPO (Antigen Aff. Pur.) (Leinco Prod. No.: T148)
Recombinant Mouse Thrombopoietin (Leinco Prod. No.: T220)
Recombinant Mouse TPO R (Leinco Prod. No.: T377)
Recombinant Human TPO (Leinco Prod. No.: T386)
Anti-Human TPO (Leinco Prod. No.: T432)
Anti-Mouse TPO-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T492)
Recombinant Human TPO (Leinco Prod. No.: T155)
Anti-Mouse TPO (Leinco Prod. No.: T441)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
