产品编号:T185
产品描述:Anti-Human CCL25-Biotin
反应种属:human
实验方法:WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C Motif) Ligand 25 (CCL25)
Biotin
(SCYA25, TECK, Ckb15)
Polyclonal Antibody
Prod. No.: T185
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 25 (CCL25) recognizes Human CCL25. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human TECK
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human CCL25 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CCL25 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is T165.
References
1. Vacari et al. (1997) Immunity 7: 291
2. Agace W et al. (2006) J Immunol. 176: 3642
3. Teixidó J. et al. (2007) J Leukoc Biol. 82: 380
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL25 (Antigen Aff. Pur.) (Leinco Prod. No.: T167)
Recombinant Mouse TECK (Leinco Prod. No.: T396)
Anti-Human CCL25 (Leinco Prod. No.: C1399)
Anti-Human CCL25 (Leinco Prod. No.: T216)
Anti-Mouse CCL25 (Leinco Prod. No.: C1428)
Anti-Mouse CCL25 (Leinco Prod. No.: C1429)
Anti-Mouse CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T491)
Recombinant Human CCL25 (Leinco Prod. No.: T165)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
