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Anti-Human CCL17-Biotin

产品编号:T172

产品描述:Anti-Human CCL17-Biotin

反应种属:human

实验方法:WB, ELISA.

标记:

规格:50ug

供应商:leinco

价格(RMB):5700.00

订购:订购

说明书:

Goat Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17)
Biotin
(Thymus and Activation Regulated Chemokine (TARK), A-152E5.3, ABCD-2, MGC138271, MGC138273, SCYA17, TARC, Dendrokine)
Polyclonal Antibody
Prod. No.: T172
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C

Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17) recognizes Human CCL17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).3 It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.4

Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human CCL17
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Western Blotting: To detect Human TARC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TARC is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Yoshie O et al. (1996) J Biol Chem. 271: 21514
2. Yoshie O et al. (1997) J Biol Chem. 272: 15036
3. Naoyuki Kamatani et al. (2003) J Rheumatology 30: 2369
4. Kunihiko K et al. (2006) Eur J Immunol. 36: 2116

Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL17 (Leinco Prod. No.: C1404)
Anti-Mouse CCL17 (Leinco Prod. No.: C1435)
Anti-Mouse CCL17 (Leinco Prod. No.: T443)
Anti-Mouse CCL17 (Antigen Aff. Pur.) (Leinco Prod. No.: T464)
Anti-Mouse CCL17-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1510)
Recombinant Human CCL17 (Leinco Prod. No.: T158)
Anti-Human CCL17 (Antigen Aff. Pur.) (Leinco Prod. No.: T166)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.