产品编号:F152

产品描述:Anti-Human FGF-10-Biotin

反应种属:human

实验方法:WB

标记:

规格:50ug

供应商:leinco

价格(RMB):5700.00

订购:

说明书:

Goat Anti-Human Fibroblast Growth Factor-10 (FGF-10)
Biotin
(FGFA, Keratinocyte growth factor-2, Repifermin)
Polyclonal Antibody

Prod. No.: F152
Host:         Goat
Pkg. Size: 1 mg, 50 µg
Storage:      -20°C to -70°C

Description
Specificity:
Goat Anti-Human Fibroblast Growth Factor-10 (FGF-10) recognizes Human FGF-10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Fibroblast growth factor 10, also known as FGF10, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is a primary factor in the process of wound healing similarly to other growth factors such as TGF alpha and FGF7.1

Immunogen
E. coli-Derived Recombinant Human FGF-10
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human FGF-10 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rhFGF-10 is approximately 50 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

References
1. Nakatsuka M. et al. (1997) Gene197: 399
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human FGF-10 (Leinco Prod. No.: F124)
Anti-Human FGF-10 (Antigen Aff. Pur.) (Leinco Prod. No.: F151)
Recombinant Human FGF-10 (Leinco Prod. No.: K131)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.