产品编号:H256
产品描述:Anti-Human CCL14a-Biotin
反应种属:human
实验方法:WB, ELISA.
标记:
规格:50ug
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C Motif) Ligand 14 (CCL14)
Biotin
(SCYA14, HCC-1, HCC-3, NCC-2, SCYL2, CKb1, MCIF)
Polyclonal Antibody
Prod. No.: H256
Host: Goat
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 14 (CCL14) recognizes Human CCL14. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 14 (CCL14), also known as HCC-1, is a small cytokine belonging to the CC chemokine family. It is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut.1 CCL13 activates monocytes, but does not induce their chemotaxis. CCL14 is of recent interest because of its natural ability to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5).2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human HCC-1 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
References
1. Schulz-Knappe et al. (1996) J Exp Med. 183: 295
2. Choe S et al. (2007) Biochemistry 46: 10008
Related Products
Anti-Human CCL14 (Antigen Aff. Pur.) (Leinco Prod. No.: H258)
Recombinant Human CCL14 (Leinco Prod. No.: H257)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
