产品编号:B421
产品描述:Anti-Human BMP-2-Biotin
反应种属:human
实验方法:ELISA, WB
标记:
规格:50ug
供应商:leinco
价格(RMB):5625.00
订购:
说明书:
Rabbit Anti-Human Bone Morphogenetic Protein-2 (BMP-2)
Biotin
(BMP-2A, OP-1)
Polyclonal Antibody
Prod. No.: B421
Host: Rabbit
Pkg. Size: 1 mg, 50 µg
Storage: -20°C
Description
Specificity:
Rabbit Anti-Human Bone Morphogenetic Protein-2 (BMP-2) recognizes Human Bone Morphogenetic Protein-2. This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography.
Background:
BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
Human and mouse BMP-2 and BMP-7 are 100% and 98% identical, respectively, at the amino acid level. Human BMP-2 shares 85% aa sequence identity with human BMP-4 and less than 51% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60% - 70% aa sequence identity with BMP-5, -6, and -8, and less than 50% aa sequence identity with other BMPs. BMP-2 and BMP-7 are co-expressed in some embryonic tissues 6,7 and associate into a functional 38 kDa osteogenic dimer.8 In in vitro osteoblast differentiation assays and in vivo bone formation models, a BMP-2/BMP-7 heterodimer is significantly more potent than either homodimer.
Bone morphogenetic protein 2 is shown to stimulate the production of bone and recombinant human protein (rhBMP-2) and is currently available for orthopaedic usage in the United States. Implantation of BMP-2 in a collagen sponge induces new bone formation and can be used for the treatment of bony defects, delayed union, and non-union. Bone morphogenetic protein 2 has also found its way into the field of Dentistry. Oral Surgery and Implant Dentistry in particular have benefited dramatically from commercially available BMP-2.
Immunogen
Purified Recombinant Human BMP-2 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
This biotinylated antigen affinity purified polyclonal antibody should be aseptically reconstituted in 500 µl of sterile water containing 0.1% BSA to a final concentration of.
Storage and Stability
This lyophilized biotinylated polyclonal antibody should be stored desiccated at -20°C. The reconstituted conjugate is stable for at least one month when stored at 2 - 8°C. For long term storage aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid More Than One Freeze Thaw Cycle.
Reported Applications
WB
Western Blotting: To detect Human BMP-2 this biotin conjugate can be used at a concentration of 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human BMP-2 is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human BMP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: B420)
Recombinant Human BMP-2 (Leinco Prod. No.: B388)
Anti-Human BMP-2 (Leinco Prod. No.: B576)
Anti-BMP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B610)
Human BMP-2 ELISA Development Kit (Leinco Prod. No.: B422)
Recombinant Human BMP-2 (Leinco Prod. No.: B625)
Anti-Human BMP-2 (Leinco Prod. No.: B649)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
