产品编号:E134
产品描述:Anti-Mouse EGF
反应种属:mouse
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Epidermal Growth Factor (EGF)
Antigen Affinity Purified
(Urogastrone, URG, C-erbB)
Polyclonal Antibody
Prod. No.: E134
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Mouse Epidermal Growth Factor (EGF) recognizes Mouse EGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR.1 EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. This causes variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.2 EGF has been shown to regulate tumor cell invasion through activation of matrix metalloproteinase-2 (MMP-2) in various tumor cell types.3
Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse EGF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse EGF is approximately 0.5 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Mouse EGF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse EGF (at 0.3 ng/ml) is 0.02 - 0.06 µg/ml.
Western Blotting: To detect Mouse EGF this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse EGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Cohen S et al. (1995) J. Biol. Chem 265: 7709
2. Seroogy KB et al. (1995) Science 224: 1107
3. Cosen-Binker LI. et al. (2009) Biochem Biophys Res Commun. 379: 445
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse EGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E135)
Recombinant Rat EGF (Leinco Prod. No.: E188)
Anti-Human EGF (Leinco Prod. No.: E239)
Anti-Human EGF (Leinco Prod. No.: E256)
Anti-Human EGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E276)
Anti-Human EGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E121)
Human EGF ELISA Development Kit (Leinco Prod. No.: E141)
Mouse EGF ELISA Development Kit (Leinco Prod. No.: E305)
Anti-Human EGF (Antigen Aff. Pur.) (Leinco Prod. No.: E104)
PN:
Recombinant Mouse EGF (Leinco Prod. No.: E120)
Recombinant Human EGF (Leinco Prod. No.: E108)
Anti-Rat EGF ( Antigen Aff. Pur. ) (Leinco Prod. No.: E315)
Anti-Rat EGF- Biotin ( Antigen Aff. Pur. ) (Leinco Prod. No.: B636)
Anti-Mouse EGF (Leinco Prod. No.: E321)
Anti-Rat EGF (Leinco Prod. No.: E323)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
