产品编号:C399
产品描述:Anti-Mouse CTLA-4
反应种属:mouse
实验方法:ELISA, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Mouse Cytotoxic T-Lymphocyte Antigen-4 (CTLA-4)
Antigen Affinity Purified
(CD152, CELIAC3, GSE, IDDM12)
Polyclonal Antibody
Prod. No.: C399
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Mouse Cytotoxic T-Lymphocyte Antigen-4 (CTLA-4) recognizes Mouse CTLA-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system.1 CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells.2,3 CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment.
Known Species Cross Reactivity: Ms+
Immunogen
NS0-Derived Recombinant Mouse CTLA-4 Extracellular Domain
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.2 - 0.8 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505).
Western Blotting: To detect Mouse CTLA-4 this polyclonal antibody can be used at a concentration of 0.1 – 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rmCTLA-4 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Layrisse Z et al. (2009) Hum Immunol.
2. Mak TW et al. (1995) Science 270: 985
3. Delneste Y et al. (1999) Eur. J. Immunol 29: 3596
4. Vijayakrishnan L et al. (2004) Immunity 20: 563
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CTLA-4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C400)
Recombinant Human CTLA-4 (Leinco Prod. No.: C1319)
Recombinant Mouse CTLA-4 (Leinco Prod. No.: C1340)
Anti-Mouse CTLA-4-Fluorescein (FITC) (Leinco Prod. No.: C1582)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
