产品编号:T191
产品描述:Anti-Human TWEAK
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human TNF-Related Weak Inducer of Apoptosis (TWEAK)
Antigen Affinity Purified
(TNFSF12, DR3LG, Apo3-Ligand)
Polyclonal Antibody
Prod. No.: T191
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human TNF-Related Weak Inducer of Apoptosis (TWEAK) recognizes Human TWEAK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
TNF-related weak inducer of apoptosis (TWEAK) is a member of the TNF ligand family1 that has pleiotropic biological functions including proapoptotic, proangiogenic and proinflammatory activities.2 TWEAK induces skeletal muscle atrophy.3 TWEAK functions both as a type II transmembrane protein and as a cleaved soluble protein. TWEAK transcripts are expressed in heart, brain, skeletal muscle, pancreas, immune system, ovary, and placenta.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human TWEAK (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human TWEAK is approximately 0.2-0.3 ng/well.
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 10 µg/ml to detect human TWEAK in MDA-MB-231 cells.
Neutralization: This antibody is useful for neutralization of Human TWEAK bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TWEAK (at 1 ng/ml) is 2-8 µg/ml.
Western Blotting: To detect Human TWEAK this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TWEAK is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:.
References
1. Burkly LC et al. (2005) Oncogene 24: 2613
2. Young Lee S. et al. (2003) Biochem Biophys Res Commun. 305: 789
3. Kumar A et al. (2007) FASEB J. 21: 1857
4. Aaron J. W. Hsueh et al. (2006) Molecular Endo. 20: 2528
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human TWEAK-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T197)
Anti-Human TWEAK (Leinco Prod. No.: T309)
Recombinant Human TWEAK (Leinco Prod. No.: T169)
Human TWEAK ELISA Development Kit (Leinco Prod. No.: T514)
Recombinant Mouse TWEAK (Leinco Prod. No.: T524)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
