产品编号:T147
产品描述:Anti-Human TNF-β
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Tumor Necrosis Factor-Beta (TNF-β)
Antigen Affinity Purified
(Lymphotoxin-Alpha (LT Alpha), Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha 3)
Polyclonal Antibody
Prod. No.: T147
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Tumor Necrosis Factor-Beta (TNF-β) recognizes Human TNF-β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Tumor necrosis factor-beta (TNF beta) also known as lymphotoxin alpha (LT alpha), is a multifaceted pro-inflammatory cytokine which may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.1 TNF beta has been implicated in the pathogenesis of several autoimmune diseases and it plays a significant role in the etiopathogenesis of multiple sclerosis (MS).2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human TNF-β (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
References
1. Adolf GR et al. (1990) Infec Immun. 58: 3996
2. Pandey JP et al. (2002) Autoimmunity. 35: 377
Related Products
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Anti-Human TNF-β (Leinco Prod. No.: T546)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
