产品编号:T145
产品描述:Anti-Human TGF-α
反应种属:human
实验方法:ELISA, IHC-F, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Transforming Growth Factor-Alpha (TGF-α)
Antigen Affinity Purified
(Sacroma Growth Factor, TGF-Type I, ETGF, MDGF-2 (Milk-Derived Growth Factor-2), TCGF (Transformed Cell Growth Factor))
Polyclonal Antibody
Prod. No.: T145
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Transforming Growth Factor-Alpha (TGF-α) recognizes Human TGF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Transforming growth factor alpha (TGF-α) is thought to be the major autocrine factor controlling growth in epidermal cells.1 It plays an important role in cell proliferation during embryogenesis.2 It also stimulates neural cell proliferation in the adult injured brain.3 TGF-alpha is an oncogenic growth factor which drives estrogen independent breast tumor proliferation via an autocrine loop.4 TGF-alpha which normally suppresses tumours can actually be used by cancer cells to enhance their ability to spread. It is present in high levels in around half of all breast tumours, and these tumours tend to be more aggressive and more likely to spread to the lungs. It has been shown that TGF-alpha can prompt breast cancer cells to make another cytokine called angiopoietin-like 4 (ANGPTL4), which helps the cells to spread through the bloodstream.5
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human TGF-α (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is Anti-Human TGF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T186) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human TGF-α (Leinco Prod. No.: T151).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human TGF-α this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TGF-α is 50 and 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human TGF-α (Leinco Prod. No.: T151).
References
1. Fuchs E et al. (1991) Genes & Dev. 5: 714
2. Lamaroon A et al. (2002) J Dental Research 75: 1534
3. Loughlin S et al. (2002) Proc Natl Acad Sci USA 97: 14686
4. Taylor K et al. (1997) Medicine
5. (2008) Cancer Research UK
Related Products
Anti-Human TGF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T186)
Recombinant Human TGF-α (Leinco Prod. No.: T151)
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
