产品编号:T166
产品描述:Anti-Human CCL17
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17)
Antigen Affinity Purified
(Thymus and Activation Regulated Chemokine (TARC), A-152E5.3, ABCD-2, MGC138271, MGC138273, SCYA17, TARC, Dendrokine)
Polyclonal Antibody
Prod. No.: T166
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17) recognizes Human CCL17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).3 It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.4
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human TARC (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human CCL17 is approximately 0.1 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human CCL17 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL17 (at 0.01 ng/ml) is 0.3-1.5 µg/ml.
Western Blotting: To detect Human CCL17 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CCL17 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human CCL17 (Leinco Prod. No.: T158).
References
1. Yoshie O et al. (1996) J Biol Chem. 271: 21514
2. Yoshie O et al. (1997) J Biol Chem. 272: 15036
3. Naoyuki Kamatani et al. (2003) J Rheumatology 30: 2369
4. Kunihiko K et al. (2006) Eur J Immunol. 36: 2116
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human CCL17 (Leinco Prod. No.: T158)
Anti-Human CCL17 (Leinco Prod. No.: C1404)
Anti-Mouse CCL17 (Leinco Prod. No.: C1435)
Anti-Mouse CCL17 (Leinco Prod. No.: T443)
Anti-Mouse CCL17 (Antigen Aff. Pur.) (Leinco Prod. No.: T464)
Anti-Mouse CCL17-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1510)
Anti-Human CCL17-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T172)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
