产品编号:P122
产品描述:Anti-Human PDGF-BB
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Platelet Derived Growth Factor-BB (PDGF-BB)
Antigen Affinity Purified
(Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF))
Polyclonal Antibody
Prod. No.: P122
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Platelet Derived Growth Factor-BB (PDGF-BB) recognizes Human PDGF-BB. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.1 This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.2 PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.3 PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.4 PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.5 It may be a new prognostic indicator for patients with colorectal cancer.6 PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.5
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human PDGF-BB (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
References
1. Josef Pfeilschifter et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada N et al. (1992) J Biol Chem. 267: 15455
4. Mustoe TA et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori M et al. (2008) Ann Surg Oncol. 15: 2129
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
