产品编号:N147
产品描述:Anti-Human CLC
反应种属:human
实验方法:ELISA, ELISA, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Cardiotrophin-Like Cytokine (CLC)
Antigen Affinity Purified
(NR6, B Cell Stimulating Factor-3 (BSF3), Novel Neurotrophin-1 (NNT1), CISS2, CLCF1)
Polyclonal Antibody
Prod. No.: N147
Host: Goat
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Novel Neurotrophin-1 (NNT-1) recognizes Human NNT-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.1 CLC is expressed highly in spleen and peripheral leukocytes2 and is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4
Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human CLC
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human NNT-1 is approximately 1.3 ng/well.
Western Blotting: To detect Human NNT-1/CLC this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human NNT-1/CLC is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Taga T et al. (2002) Cytokine 18: 1
2. Shi Y et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim RW et al. (2003) J Neurosci. 23: 8854
4. Rong Wen et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CLC-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N148)
Recombinant Human CLC (Leinco Prod. No.: N138)
Anti-Human CLC (Leinco Prod. No.: C1462)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
