产品编号:I-145

产品描述:Anti-Human IL-1β

反应种属:human

实验方法:ELISA, IHC-F, N, WB

标记:

规格:100ug

供应商:leinco

价格(RMB):5595.00

订购:

说明书:

Goat Anti-Human Interleukin-1 Beta (IL-1β)
Antigen Affinity Purified
(IL1B, IL-1, IL1-Beta, IL1F2)
Polyclonal Antibody

Prod. No.: I-145
Host:          Goat
Pkg. Size: 1 mg, 100 µg
Storage:       -20°C to -70°C

Description
Specificity:
Rabbit Anti-Human Interleukin-1 Beta recognizes Human Interleukin-1 Beta. This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography.

Background:
Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes.1,2 IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human Interleukin-1 Beta (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.01 EU/µg as determined bythe LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB

References
1. Dinarello CA et al. (1996) Blood 87: 2095
2. Yuan et al. (1993) Cell 75: 641
3. Shin Maeda et al. (2005) Science 4: 735

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.