产品编号:I-143
产品描述:Anti-Human IGF-II
反应种属:human
实验方法:ELISA, IHC, N, WB
标记:
规格:250ug
供应商:leinco
价格(RMB):5595.00
订购:
说明书:
Goat Anti-Human Insulin Like Growth Factor-II (IGF-11)
Antigen Affinity Purified
(Somatamedin A, Somatomedin C, C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974)
Polyclonal Antibody
Prod. No.: I-143
Host: Goat
Pkg. Size: 1 mg, 250 µg
Storage: -20°C to -70°C
Description
Specificity:
Rabbit Anti-Human Insulin Like Growth Factor-II (IGF-11) recognizes Human IGF-11. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.1 IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.2
Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IGF-11 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.25 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
References
1. A. Hoeflich et al. (1994) Hormone Research 41: 66
2. Andrée Gruslin et al. (2005) The National Acad. of Sci.
Related Products
Recombinant Human IGF-II (Leinco Prod. No.: I-181)
Anti-Human IGF-II-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-241)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
