产品编号:C1217
产品描述:Anti-Human CARMA1
反应种属:mouse, human
实验方法:IHC-F, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human CARMA1
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: C1217
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human CARMA1 recognizes an epitope near the C-terminus of human and mouse CARMA1. This polyclonal antibody was purified using affinity chromatography.
Background:
CARMA proteins belong to the membrane-associated guan-ylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules.1 CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types.2,3 Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the I/K complex, thereby regulating activation of the NF-κB transcription factor.5 Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.6
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
CARMA1 Blocking Peptide (Leinco Prod. No.: C1231)
Blocking Peptide
CARMA1 Blocking Peptide (Leinco Prod. No.: C1231)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB
Western Blotting: This antibody can be used to detect human and mouse CARMA1 by Western blot analysis at a concentration of 0.5-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Human Thymus Tissue Lysate (Leinco Prod. No.: H1431) or Mouse Thymus Tissue Lysate (Leinco Prod. No.: M1158).
References
1. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure.Curr. Opin. Cell Biol. 1999; 11:432-9.
2. Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-κ B activation. FEBS Lett. 2001; 496:121-7.
3. Bertin J, Wang L, Guo Y, et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-κ B. J. Biol. Chem. 2001; 276:11877-82.
4. McAllister-Lucas LM, Inohara N, Lucas PC, et al. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-κB induction. J. Biol. Chem. 2001; 276:30589-97.
Related Products
CARMA1 Blocking Peptide (Leinco Prod. No.: C1231)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Thymus Tissue Lysate (Leinco Prod. No.: H1431)
Mouse Thymus Tissue Lysate (Leinco Prod. No.: M1158)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
