产品编号:C1215
产品描述:Anti-Human CARD10(CT)
反应种属:mouse, human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Caspase Recruitment Domain Family Member 10 (CARD10)
Purified No Carrier Protein
(CARMA3, Bimp1)
Polyclonal Antibody
Prod. No.: C1215
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Caspase Recruitment Domain Family Member 10 (CARD10) recognizes an epitope near the C-terminus of human and mouse CARD10. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10.1 This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules.2 CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB.1,3 CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex,4 demonstrating its importance in the regulation of NF-κB transcription factor activation.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
CARD10 (CT) Blocking Peptide (Leinco Prod. No.: C1227)
Blocking Peptide
CARD10 (CT) Blocking Peptide (Leinco Prod. No.: C1227)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and mouse CARD10 by Western blot analysis at a concentration of 5-10 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is EL4 Whole Cell Lysate (Leinco Prod. No.: E153).
References
1. Wang L, Guo Y, Huang W-J, et al. CARD10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-κB. J. Biol. Chem. 2001; 276:21405-9.
2. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
3. Fischer KD, Tedford K, Wirth T. New roles for Bcl10 in B-cell development and LPS response. Trends Immunol. 2004; 25:113-6.
4. Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with Iκkinase κ-NF-κB essential modulator. J. Biol. Chem. 2004; 279:34323-31.
Related Products
CARD10 (CT) Blocking Peptide (Leinco Prod. No.: C1227)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
EL4 Whole Cell Lysate (Leinco Prod. No.: E153)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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