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Anti-Human BID(CT)

产品编号:B455

产品描述:Anti-Human BID(CT)

反应种属:mouse, human

实验方法:IHC, WB

标记:

规格:0.1mg

供应商:leinco

价格(RMB):5370.00

订购:订购

说明书:

Rabbit Anti-Human BID
Purified No Carrier Protein
(Caspase-8-Cleaved)
Polyclonal Antibody

Prod. No.: B455
Host:        Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:    2-8°C

Description
Specificity:
Rabbit Anti-Human BID recognizes an epitope near the C-terminus of human and mouse Bid. This polyclonal antibody was purified using affinity chromatography.

Background:

Bid is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane. Cleavage of Bid by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria. Bid may then interact with another pro-apoptotic Bcl-2 family member Bak. Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A 9 - 13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti-apoptotic member Bcl-x.

Known Species Cross Reactivity: Ms+, Hu+

Immunogen
BID (CT) Blocking Peptide (Leinco Prod. No.: B486)
Blocking Peptide
BID (CT) Blocking Peptide (Leinco Prod. No.: B486)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB

References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.

3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.

4. Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.
Related Products
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Anti-Human BID (Intermediate Domain) (Leinco Prod. No.: B456)
Anti-Human/Mouse BID-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B617)
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Recombinant Human BID (Leinco Prod. No.: B550)
Recombinant Human BID (Leinco Prod. No.: B553)
Recombinant Mouse BID (Leinco Prod. No.: B549)
Anti-Human BID ( Anitgen Aff. Pur. ) (Leinco Prod. No.: B644)
Anti-Human/Mouse BID ( Antigen Aff. Pur. ) (Leinco Prod. No.: B645)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.