产品编号:A251
产品描述:Anti-Human ASK1(NT)
反应种属:mouse, human
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Apoptosis signal-regulating kinase (ASK1)
Purified No Carrier Protein
(MAPKKK, MEK kinase 5)
Polyclonal Antibody
Prod. No.: A251
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Apoptosis signal-regulating kinase (ASK1) recognizes an epitope near the N-terminus of human and mouse ASK1. This polyclonal antibody was purified using affinity chromatography.
Background:
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5.1-3 ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-Α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Apoptosis Signal-Regulating Kinase (NT) Blocking Peptide (Leinco Prod. No.: A265)
Blocking Peptide
Apoptosis Signal-Regulating Kinase (NT) Blocking Peptide (Leinco Prod. No.: A265)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect human and mouse ASK1 by Western blot analysis at a concentration of 1-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PN:A231.
References
1. ASK1 antibody can be stored at 4˚C, stable for one year.1. Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997; 275:90-4.
2. Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem. 1996; 271:31607-11.
3. Tobiume K, Inage T, Takeda K, et al. Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.M Biochem. Biophys. Res. Commun. 1997; 239:905-10.
Related Products
Apoptosis Signal-Regulating Kinase (NT) Blocking Peptide (Leinco Prod. No.: A265)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PN:A231
Anti-Human Apoptosis signal-regulating kinase (CT-1) (ASK1) (Leinco Prod. No.: A158)
Anti-Human Apoptosis signal-regulating kinase (CT-2) (ASK1) (Leinco Prod. No.: A250)
Apoptosis Signal-Regulating Kinase (CT-2) Blocking Peptide (Leinco Prod. No.: A264)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
