产品编号:A246
产品描述:Anti-Human AIM(NT)
反应种属:mouse, human
实验方法:WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Apoptosis Inhibitor of Macrophages (AIM)
Purified No Carrier Protein
(API6, SP alpha, CD5L)
Polyclonal Antibody
Prod. No.: A246
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Apoptosis Inhibitor of Macrophages (AIM) recognizes an epitope near the N-terminus of human and mouse AIM. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis inhibitor of macrophages (AIM) is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that AIM functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. AIM was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant AIM significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that AIM functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Apoptosis Inhibitor of Macrophages (NT) Blocking Peptide (Leinco Prod. No.: A258)
Blocking Peptide
Apoptosis Inhibitor of Macrophages (NT) Blocking Peptide (Leinco Prod. No.: A258)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human AIM by Western blot analysis at a concentration of 1-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Lymph Node Normal Tissue Lysate (Leinco Prod. No.: L161).
References
1. Miyazaki T, Hirokami Y, Matsuhashi N, et al. Increased susceptibility of thymocytes to apoptosis in mice lacking AIM, a novel murine macrophage-derived soluble factor belonging to the scavenger receptor cysteine-rich domain superfamily. J. Exp. Med. 1999; 189:413-22.
2. Biancone L, Bowen MA, Lim A, et al. Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes. J. Exp. Med. 1996; 184:811-9.
3. Haruta I, Kato Y, Hashimoto E, et al. Association of AIM, a novel apoptosis inhibitory factor, with hepatitis via supporting macrophage survival and enhancing phagocytic function of macrophages. J. Biol. Chem. 2001; 276:22910-4
4. Kuwata K, Watanabe H, Jiang S-Y, et al. AIM inhibits apoptosis of T cells and NKT cells in Corynebacterium-induced granuloma formation in mice. Am. J. Path. 2003; 162:837-47.
Related Products
Apoptosis Inhibitor of Macrophages (NT) Blocking Peptide (Leinco Prod. No.: A258)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Lymph Node Normal Tissue Lysate (Leinco Prod. No.: L161)
Anti-Human Apoptosis Inhibitor of Macrophages (AIM) (CT) (Leinco Prod. No.: A245)
Apoptosis Inhibitor of Macrophages (CT) Blocking Peptide (Leinco Prod. No.: A257)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
