产品编号:LD-1211

产品描述:Human Cardiac Troponin-I, Grade 2

反应种属:Human

实验方法:

标记:

规格:1 mg

供应商:Life Diagnostics

价格(RMB):15400.00

订购:

说明书:

Human Cardiac Troponin-I
Catalog No.              1211
Analysis                     SDS PAGE: Minor 24 kDa band with multiple low molecular weight fragmentation bands.
Western Blot             All bands on SDS PAGE reactive with Tn-I MAb.
Purity                       95%
Concentration            0.25- 2 mg/ml
Buffer                           8 M Urea, 50 mM Tris.HCl, 15 mM b-mercaptoethanol, 1 mM EDTA (pH 8.0)
Storage                       -20oC (avoid repeated freeze thawing)
Source                         Human cardiac tissue
Troponin I (TnI) is one of three subunits that form the troponin complex of the thin
filaments of striated muscle. The troponin complex serves as a calcium-sensitive switch
that regulates striated muscle contraction. Troponin I binds actin and inhibits actomyosin
ATPase activity in the absence of calcium. Cardiac troponin I is characterized by a
molecular weight of ~24 kDa. It is expressed only in the heart and has proved to be a
specific biomarker for heart disease. Cardiac troponin-I is purified by ion-exchange
chromatography in conjunction with calcium dependent affinity chromatography on
troponin-C agarose. This partially degraded form of cardiac troponin-I can be used with
good success as an immunogen for preparation of troponin-I antibodies.