Contents
The vial normally contains 3-5 µg purified medaka vitellogenin (Vtg). The content of each vial may vary from batch to batch, see exact amount on the label of the vial.

Source of vitellogenin
Japanese medaka (Oryzias latipes) induced with 17ß-estradiol.

Purification procedure
Medaka Vtg was purified from whole body homogenate of 17ß-estradiol-induced fish by ion exchange chromatography and gel filtration (1).

Application
The lyophilized medaka Vtg may be used as a positive control in western blot and ELISA. Freshly reconstituted Vtg may also be used as a standard in a quantitative ELISA.

Storage
Lyophilized vitellogenin can be stored at 4oC. We recommend reconstitution in 300-1000 µl cold PBS immediately before use. Do not freeze and thaw if Vtg is used as a quantitative standard. For use only as a positive control the solution may be aliquoted and stored at -20oC. Avoid repeated freezing and thawing.
Note: If the solution of vitellogenin after reconstitution apperars turbid, add 1-2 µl of 0.2 M EDTA, pH 7,7 until the solution becomes clear.

References
1) Brion, F., Nilsen, B.M., Eidem, J.K., Goksøyr, A., and Porcher,J-M. (2002) Environ Toxicol Chem. 28, 1699-1708.

For research use only.