产品编号:V01407101-100

产品描述:Monoclonal mouse anti-killifish vitellogenin- ND-5F8

反应种属:

实验方法:

标记:

规格:100ul

供应商:Biosense

价格(RMB):5000.00

订购:

说明书:

Monoclonal mouse anti-killifish vitellogenin

Subclass/Isotype:
IgG1-kappa.

Contents:
This product consists of Protein-G affinity-purified mouse monoclonal antibodies against vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus) . The antibodies are dissolved in 0.1 M Na-phosphate, 0.15 M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.

Immunogen source:
Vitellogenin purified from plasma of 17ß-estradiol-treated killifish, white perch and bowfin (Amia calva). Egg yolk from swordfish (Xiphias gladius).

Specificity:
ND-5F8 binds with high affinity to vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). The antibody also cross-reacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). The antibody does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius). The degree of cross reactivity differs between species and with the different methods employed.

Application:
ND-5F8 works well in both ELISA and immunoblotting for a number of species. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.

Normal dilution range:
ELISA: 1:100 - 1:1000
Western blot: 1:100 - 1:500

Storage:
In the dark at 4°C

For research use only.