产品编号:C02401101-500
产品描述:Monoclonal mouse anti-fish CYP1A-7
反应种属:
实验方法:
标记:
规格:500ul
供应商:Biosense
价格(RMB):10875.00
订购:
说明书:
Monoclonal mouse anti-fish CYP1A
Subclass/Isotype:
IgG3-kappa.
Contents:
The product consists of Protein-A affinity-purified mouse monoclonal antibodies against peptide 277-294 of rainbow trout (Oncorhynchus mykiss) cytochrome P450 1A (CYP1A). The antibodies are dissolved in 0.1 M Na-phosphate, 0.15 M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.
Immunogen source:
A synthetic peptide corresponding to amino-acid sequence 277-294 of rainbow trout CYP1A, coupled to the carrier protein keyhole limpet hemocyanin (KLH).
Specificity:
C10-7 cross-reacts with CYP1A in liver samples from carp (Cyprinus carpio), rainbow trout (Oncorhynchus mykiss), salmon (Salmo salar), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus) lemon sole (Microstomus kitt) and mudfish (Clarias anguillaris). The degree of cross-reactivity differ between species and with the different methods employed.
Applications:
C10-7 is well suited for ELISA. The antibody also work well in western blot for a number of species. The antibody also works well in immunohistochemistry. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
Normal dilution range:
ELISA: 1:300 - 1: 1000
Western blot: 1:50 - 1:500
Storage:
In the dark at 4°C.
For research use only.
References:
