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Test Principle
The test is a direct competitive ELISA that allows the detection of Cylindrospermopsin. It is based on
the recognition of Cylindrospermopsin by specific antibodies. Cylindrospermopsin, when present in a
sample, and a Cylindrospermopsin-HRP analogue compete for the binding sites of rabbit anticylindrospermopsin
antibodies in solution. The cylindrospermopsin antibodies are then bound by a
second antibody (sheep anti-rabbit) immobilized in the plate. After a washing step and addition of the
substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to
the concentration of the Cylindrospermopsin present in the sample. The color reaction is stopped after a
specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are
determined by interpolation using the standard curve constructed with each run.
